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用聚合酶链反应（ＰＣＲ－ＥＢ）技术，对ＨＢｓＡｇ阳性孕妇７８例（实验组）和ＨＢｓＡｇ阴性孕妇４３例（对照组）的血清、脐血和乳汁中的ＨＢＶ－ＤＮＡ进行检测，同时用酶联免疫吸附法（ＥＬＩＳＡ）检测两组孕妇血清ＨＢＶ标志物，结合孕妇感染状态做相关性分析。结果：实验组血清ＨＢＶＤＮＡ检出率为５１．３％，对照组未检出，两组有极显著性差异（Ｐ＜０．００１）。实验组乳汁、脐血中ＨＢＶ－ＤＮＡ阳性率分别为２９．５％、３９．７％，对照组未检出，两组有极显著性差异（Ｐ＜０．００１）。提示母婴ＨＢＶ垂直传播与孕妇ＨＢＶ感染状态和ＨＢＶ－ＤＮＡ的检出率有关
HBV-DNA in serum, umbilical blood and milk of 78 HBsAg-positive pregnant women and 43 pregnant women with HBsAg-negative pregnant women (control group) were detected by polymerase chain reaction (PCR-EB) The serum HBV markers of two groups of pregnant women were detected by ELISA, and the correlation with the infection status of pregnant women was analyzed. Results: The detection rate of HBVDNA in the experimental group was 51.3%, but not in the control group. There was a significant difference between the two groups (P <0.001). The positive rates of HBV-DNA in milk and umbilical cord blood of the experimental group were 29.5% and 39.7% respectively, but not in the control group. There was a significant difference between the two groups (P <0.001). Prompted the vertical transmission of maternal and infant HBV and HBV infection in pregnant women status and the detection rate of HBV-DNA