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以1M蔗糖垫超离心法制备了大鼠肝聚核蛋白体(以下简称Rp),同时从去Rp上清中又经35～50％饱和度的硫酸铵盐析和DEAE-52氯化钠梯度柱层析制备了核糖核酸酶天然抑制物(以下简称RI)。Rp经紫外光谱分析,蔗糖梯度超离心分析及麦胚无细胞体系翻译活力的测定表明制备过程中基
Rat liver polyribosomes (hereinafter referred to as Rp) were prepared by ultracentrifugation with 1 M sucrose pad and simultaneously with 35% to 50% saturation of ammonium sulfate salinization and DEAE-52 sodium chloride gradient A natural inhibitor of ribonuclease (hereinafter referred to as RI) was prepared by column chromatography. Rp analysis by ultraviolet spectroscopy, sucrose gradient ultracentrifugation and determination of translation activity of wheat germ cell-free system showed that during the preparation process