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目的:观察中药海螵蛸、骨碎补、三七、阿胶含药血清对体外培养大鼠成骨细胞增殖、分化功能的影响。方法:应用MTT法观察海螵蛸、骨碎补、三七、阿胶对体外培养成骨细胞增殖功能的影响,ELISA法观察海螵蛸、骨碎补、三七、阿胶对体外培养成骨细胞ALP含量的影响。结果:在成骨细胞的增殖方面,海螵蛸、三七含药血清对成骨细胞增殖有抑制作用,骨碎补、阿胶对成骨细胞增殖没有明显促进作用。在对成骨细胞内碱性磷酸酶(ALP)的作用方面,海螵蛸、骨碎补对体外培养大鼠成骨细胞内ALP的合成有抑制作用;三七没有明显作用;阿胶有明显的促进作用。结论:4种中药对体外培养大鼠成骨细胞增殖、分化功能的影响各不相同。海螵蛸对体外培养大鼠成骨细胞的增殖、分化功能有抑制作用;骨碎补对体外培养大鼠成骨细胞的增殖无明显作用,对其分化功能有抑制作用;三七对体外培养大鼠成骨细胞的增殖有抑制作用,对其分化功能作用不明显;阿胶对体外培养大鼠成骨细胞的增殖无促进作用,但能促进体外培养大鼠成骨细胞的分化功能。
OBJECTIVE: To observe the effects of traditional Chinese herbal medicines containing Radix Astragali, Rhizoma Drynariae, Panax notoginseng, and Ejiao on the proliferation and differentiation of rat osteoblasts in vitro. METHODS: MTT assay was used to observe the effects of J. edulis, Rhizoma Drynariae, Panax notoginseng and donkey-hide gelatin on the proliferation of osteoblasts in vitro. ELISA was used to observe the osteoblasts in vitro cultured with J. edulis, Rhizoma Drynariae, Panax notoginseng and donkeytide. The effect of ALP content. Results: In the aspect of proliferation of osteoblasts, the serum of Begonia sinensis and Panax notoginseng could inhibit the proliferation of osteoblasts. Norovirus and Ejiao could promote the proliferation of osteoblasts. In the role of alkaline phosphatase (ALP) in osteoblasts, J. edulis and Rhizoma Drynaria inhibited the synthesis of ALP in rat osteoblasts in vitro; there was no obvious effect in Panax notoginseng; enhancement. Conclusion: The effects of four kinds of Chinese herbs on the proliferation and differentiation of rat osteoblasts cultured in vitro are different. Begonia had inhibitory effects on the proliferation and differentiation of rat osteoblasts in vitro. Osteopracte had no significant effect on the proliferation of rat osteoblasts in vitro and inhibited its differentiation function. Panax pseudoginseng in vitro culture The proliferation of rat osteoblasts was inhibited and its differentiation function was not obvious. Ejiao did not promote the proliferation of rat osteoblasts in vitro, but it could promote the differentiation of rat osteoblasts in vitro.