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选取日本冲绳地区雌性蜂巢石斑鱼为对象,描述与分析了HCG与LHRHa对该鱼催产效果的差异及注射后所引起的性腺发育变化和排卵相关的性类固醇变化,希望能为蜂巢石斑鱼的人工繁殖提供可靠的理论基础,以及在实践生产中的重要参考。结果表明,HCG与LHRHa均显著促进了决定排卵的性成熟诱导物质MIS的释放以及鱼的产卵;产卵后的蜂巢石斑鱼雌二酮E2的水平显著下降,验证了蜂巢石斑鱼卵巢发育及产卵随月亮周期变化的周期性;在渔业生产中注射催产药物前,确定对象鱼卵泡发育阶段对于提高催产成功率十分重要。在LHRHa注射组,完成卵黄化的蜂巢石斑鱼催产效果最佳,卵黄化早期的鱼不产卵;HCG对卵泡发育初期的蜂巢石斑鱼的催产效果优于LHRHa,但催产效果仍不理想,而对于完成了卵黄化鱼的催产效果不如LHRHa。结合LHRHa的其他优势,本研究认为在具备检验鱼体卵巢发育阶段实验技术的条件下,更加推荐LHRHa作为雌性蜂巢石斑鱼的催产药物。
The female hive grouper in Okinawa, Japan was selected as the object to describe and analyze the differences of oxytocin-induced effects between HCG and LHRHa on the fish and the gonad development and ovulation-related sex steroid changes after injection. Artificial propagation provides a reliable theoretical basis, as well as an important reference in the practice of production. The results showed that both HCG and LHRHa significantly promoted the release of MIS, which is the sex-inducing determinant of ovulation, and the spawning of fish; the E2 level of the honeycomb grouper decreased significantly after oviposition, Development and spawning cycles with the moon cycle; before the injection of oxytocin in fisheries production, to determine the development stage of the object of the fish follicles to improve the success rate of oxytocin is very important. In the LHRHa injection group, the egg-forming honeycomb grouper produced the best ovulation effect and did not oviposit early in the egg yolk. HCG produced better results than the LHRHa in the early stage of follicular development, but the oxytocin effect was still unsatisfactory , But not as good as LHRHa for the completion of oviparous fish oxytocin. In combination with other advantages of LHRHa, this study suggests that LHRHa is more recommended as an oxytocin for female hive grouper, provided experimental techniques are available to test the developmental stage of ovary in fish.