去壁、低渗法是近年来在细胞核型分析和染色体亚显微结构上开始应用的一项新技术。它与传统的压片方法相比,获得的染色体分散性好,且比较完整。特别是对一些染色体数目多,形态又小的植物,效果更为明显。近年来,国内外一些人曾对此法在果树上的应用进行了研究,取得了一定的进展。但到目前为止,在葡萄染色体观察上的应用仍未见报道。为了给葡萄遗传和育种研 Go wall, hypotonic method in recent years in the nuclear karyotype analysis and chromosomal microstructure began to apply a new technology. Compared with the traditional tabletting method, the obtained chromosome has good dispersion and completeness. Especially for some chromosome number, shape and small plants, the effect is more obvious. In recent years, some people at home and abroad have studied the application of this method to fruit trees and made some progress. However, up to now, the application of grape chromosome observation has not been reported yet. To give grapes genetic and breeding research