作者筛选到抗旋毛虫的单克隆抗体2G_8B_(10)H_3和1B_7E_6E_9F_(12)株,抗体效价均达1:1.6×10~7。经间接ELISA鉴定,前者(2G_8)特异性强,仅与旋毛虫幼虫抗原反应,不与成虫、新生蚴抗原以及人钩虫幼虫、猪蛔虫、犬弓首线虫幼虫、日本血吸虫、卫氏肺吸虫、姜片虫其他6种蠕虫的抗原反应。用转移电泳分析2G_8的靶抗原分子量,显示其能识别幼虫排泄分泌抗原的46、53 The authors screened anti-Trichinella monoclonal antibody 2G_8B_ (10) H_3 and 1B_7E_6E_9F_ (12) strains, antibody titers reached 1: 1.6 × 10 ~ 7. The indirect ELISA confirmed that the former (2G_8) was highly specific and only reacted with Trichinella spiralis larvae antigens, but not with adult worms, nematode antigens and human hookworm larvae, Ascaris suum, Canis nematoda larvae, Schistosoma japonicum, Antigen response of six other worms of zooxanthellae. Analysis of the molecular weight of the target antigen of 2G_8 by transfer electrophoresis showed that it can recognize the excretion of antigens by larvae 46,53