作者试验了一种新的高效制备杂交瘤的技术,该技术结合了在人T细胞培养上清中鼠或人B细胞克隆扩增、辐照过的EL-4作为辅助细胞以及扩增的B细胞微电融合等方法。将分离出的T细胞悬于含10% FCS的DMEM/HAM’S F12培养液中,加5μg/ml The authors tested a novel and efficient technique for hybridoma production that incorporates amplification of mouse or human B cell clones in human T cell culture supernatants, irradiated EL-4 as a helper cell, and expanded B Cell micro-electrofusion and other methods. The isolated T cells were suspended in DMEM / HAM’S F12 medium containing 10% FCS, added with 5 μg / ml