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目的:应用分子印迹技术分离检测丹参在血清中的代谢组分。方法:采用沉淀聚合法,以丹参酮ⅡA为模板分子,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,合成丹参酮ⅡA分子印迹聚合物。用透射电子显微镜对丹参酮ⅡA分子印迹聚合物的结构、形貌进行表征;并进行吸附性试验和选择性试验研究。结果:合成的分子印迹聚合物为规则的球形,约3 h达到吸附平衡,最大吸附量为34.09μmol/g,并通过试验证明聚合物对丹参酮ⅡA具有选择性能。结论:通过分子印迹固相萃取法(MIP-SPE)与HPLC-MS联用,实现高效的分离、灵敏的检测和全面的鉴定丹参药材中多种成分及其在大鼠血清中的代谢产物。
OBJECTIVE: To separate and detect the metabolites of Salvia miltiorrhiza in serum by molecular imprinting technique. Methods: Tanshinone Ⅱ A molecular imprinting polymer was synthesized by precipitation polymerization using tanshinone ⅡA as template, ethylene glycol dimethacrylate (EGDMA) as crosslinking agent and azobisisobutyronitrile (AIBN) as initiator . The structure and morphology of tanshinone ⅡA molecularly imprinted polymer were characterized by transmission electron microscopy, and the adsorption and selectivity experiments were carried out. Results: The molecularly imprinted polymers were spherical in shape. The adsorption equilibrium was reached in about 3 h with the maximum adsorption capacity of 34.09 μmol / g. The results showed that the polymer had selectivity to tanshinone ⅡA. Conclusion: MIP-SPE and HPLC-MS are combined to achieve efficient separation, sensitive detection and comprehensive identification of various components of Radix Salviae Miltiorrhizae and their metabolites in rat serum.