近二十年来,由于新的研究技术的应用,神经解剖学有了迅速的发展。譬如,除了损毁和溃变法外,应用辣根过氧化酶(HRP)和放射自显影术(ARG)追踪神经元的传入、传出联系,应用萤光组化法显示中枢与周围神经元的单胺性质,应用萤光素双重标记法显示神经细胞轴突的侧支联系,用免疫细胞化学方法探查和确定神经元的性质、定位、分布和联系等等。电子显微镜技术(包括透射电镜、扫插电镜和冰冻蚀刻标本电镜观察)进一步在亚细胞水平揭示了神经组织的超微结构,使神经解剖学的研究跨进了新的领域。疫免电镜就是将免疫细 In the past two decades, due to the application of new research techniques, there has been a rapid development of neuroanatomy. For example, in addition to the destruction and degeneration methods, HRP and ARG were used to track the afferent and efferent connections of neurons. Fluorescent staining was used to show the relationship between the central and peripheral neurons Monoamine properties, the use of dual-labeled luciferin method to display axons of nerve cell collateral contact with the use of immunocytochemical methods to explore and determine the nature of neurons, location, distribution and contact and so on. Electron microscopy (including transmission electron microscopy, scanning electron microscopy and freeze-etched electron microscopy) further reveals the ultrastructure of nerve tissue at the subcellular level, making neuroanatomy research into new fields. Immunization is immune immune fine