本研究评价了小麦花粉粒单独培养植株的倍性水平、遗传稳定性和产量表现 ,通过根尖细胞染色体计数和叶片细胞核 DNA含量的流式细胞光度术测定两种方法对 Chris和 Chris×Sinton正反交的花粉培养植株倍性的效应 ,两种方法在小麦 H0 植株的自发加倍频率和完全可育株上都给出了大约80 %和 75%的近似结果 ,只有 1.7%是非整倍体和 16 %是单倍体。自发突变的频率也是很低的 ,Chris12 4个 DH植株只有一个含有外稃带芒的隐性突变 ,同时在 H0 代有 3个植株外表象草。花粉培养的 DH系田间所测性状直到第三代都与对照相同。 92 %和 70 %的 DH系千粒重和产量分别与对照相近。这些结果表明 ,花粉培养的 DH系在植物育种、玻璃质选择、植物改良和遗传研究中应用有很大潜力。 In this study, the ploidy, genetic stability and yield of wheat pollen grains cultured alone were evaluated. Flow cytophotometry was used to determine the chromosome counts and the nucleus DNA content of leaf apices. The effect of ploidy on pollen-cultivated plants in both reciprocal crosses gave approximate results of approximately 80% and 75% for both wheat spontaneous doubling frequency and completely fertile plants for H0 plants, with only 1.7% being aneuploid and 16% are haploid. The frequency of spontaneous mutations was also very low. Only one recessive mutation was found in the four DH12 plants of the genus Chris12 and there were three plants in the H0 generation. Pollen-cultured DH lines were measured in the field until the third generation with the same control. 92% and 70% of the DH 1000-grain weight and yield were similar to the control. These results indicate that pollen-grown DH lines have great potential for application in plant breeding, vitreous selection, plant improvement and genetic research.