多聚酶链反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）是当代分子生物学的重要技术。在对ＰＣＲ产物进行限制酶消化时，由于限制酶需一定的反应条件，往往需对ＰＣＲ产物进行纯化。我们在应用ＨｉｎｄⅢ酶切ＰＣＲ产物时，采用了纯化后酶切及直接酶切两种 Polymerase chain reaction (PCR) is an important technique in contemporary molecular biology. In the case of restriction enzyme digestion of the PCR product, the PCR product is usually purified due to the restriction enzyme needs a certain reaction condition. We use Hind Ⅲ digestion PCR products, the use of purified digestion and direct digestion of two