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目的:建立中药凌霄花中麦角甾苷、齐墩果酸、熊果酸含量测定方法。方法:色谱柱Agilent ZORBAX EclipseXDB-18,流动相甲醇-0.1%磷酸梯度洗脱,检测波长:0~30 min为334 nm,30~60 min为210 nm。结果:麦角甾苷、齐墩果酸和熊果酸3成分浓度分别在0.025 9~0.258,0.100~1.00,0.104~1.04 g.L-1与峰面积呈良好线性关系,平均加样回收率依次为98.9%,99.3%,99.4%。结论:可同时测定麦角甾苷、齐墩果酸和熊果酸含量,可用于药材质量控制。
Objective: To establish a method for the determination of acteoside, oleanolic acid and ursolic acid in Campanulaceae. Methods: The column was eluted with Agilent ZORBAX EclipseXDB-18 and mobile phase consisted of methanol-0.1% phosphoric acid. The detection wavelength was 334 nm at 0-30 min and 210 nm at 30-60 min. Results: The concentrations of acteoside, oleanolic acid and ursolic acid were 0.025 9-0.258, 0.100-1.00 and 0.104-1.04 gL-1, respectively. The average recoveries were 98.9 %, 99.3%, 99.4%. Conclusion: The content of acteoside, oleanolic acid and ursolic acid can be simultaneously determined, which can be used for the quality control of medicinal materials.