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观察恒河猴和人骨骼肌形态学的相同性及差异性。方法应用肌活检,HE染色和电镜观察恒河猴和人骨骼肌超微结构,并通过线粒体ATP酶和NADH-TR酶细胞化学染色观察恒河猴和人骨骼肌肌纤维类型的差异。结果恒河猴肌纤维直径小于人,粗细肌丝发达,与人类显著不同的是恒河猴肌纤维Ⅱ型肌更为发达(96%以上)。结论恒河猴骨骼肌组织适合于作为人类基因治疗和功能性细胞移植实验的靶组织。
To observe the homology and diversity of morphology of rhesus monkey and human skeletal muscle. Methods The ultrastructures of rhesus monkeys and human skeletal muscle were observed by muscle biopsy, HE staining and electron microscopy. The differences of muscle fiber types between rhesus monkey and human skeletal muscle were observed by cytochemical staining of mitochondrial ATPase and NADH-TR. Results Rhesus monkey muscle fiber diameter less than human, thick and thin myofilament developed significantly different from human rhesus monkey muscle fiber type Ⅱ muscle is more developed (96%). Conclusion Rhesus monkey skeletal muscle tissue is suitable as a target tissue for human gene therapy and functional cell transplantation experiments.