论文部分内容阅读
为建立一种用于检测胞内劳森菌的检测方法,本试验根据GenBank中公布的胞内劳森菌16SrRNA设计引物建立PCR诊断方法。结果显示:获得了大小为481bp的PCR产物,核苷酸序列同源性分析结果均为99%以上;特异性试验表明该方法对大肠杆菌、沙门菌、志贺菌、猪流行性腹泻病毒和金黄色葡萄球菌均为扩增阴性;敏感性试验表明该方法的最低检出量为32.8μg/L;对20份临床样品阳性检出率为15%。结果表明:该方法具有较好的特异性和敏感性,适用于临床检测胞内劳森菌。
In order to establish a detection method for detecting Lawsonia intracellularis, PCR assay was designed based on 16S rRNA of Lawsonia intracellularis published in GenBank. The results showed that the 481bp PCR product was obtained, and the nucleotide sequence homology analysis results were more than 99%. The specificity test showed that this method was effective against Escherichia coli, Salmonella, Shigella, Porcine Epidemic Diarrhea virus and Staphylococcus aureus were negative for amplification; the sensitivity test showed that the minimum detectable amount of this method was 32.8μg / L; the positive detection rate was 15% for 20 clinical samples. The results showed that this method has good specificity and sensitivity and is suitable for the clinical detection of Lawsonia intracellularis.