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乳腺癌中雌激素受体(ER)和孕酮受体的含量与内分泌治疗效应密切相关。因而在治疗乳腺癌时,对这两种受体的测定极为重要。本文介绍一种快速灵敏,且一次可分析多达10种肿瘤样品的简便方法。用兔子宫作对照,制备胞浆液,并稀释至蛋白质浓度为1～0.062mg/ml;用~(125)I-雌二醇温孵,并用右旋糖苷包裹的活性碳吸附,以去除游离的放射标记的雌二醇。样品用乙烯雌酚
The content of estrogen receptor (ER) and progesterone receptor in breast cancer is closely related to the effect of endocrine therapy. Therefore, in the treatment of breast cancer, the determination of these two receptors is extremely important. This article presents a quick and easy way to analyze up to 10 tumor samples at a time. The rabbit uterus was used as a control to prepare a cytosol and diluted to a protein concentration of 1 to 0.062 mg / ml; it was incubated with 125 I-estradiol and dextran-coated activated carbon was adsorbed to remove the free Of radiolabeled estradiol. Sample with diethylstilbestrol