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厦门市同安地区已成为中国第二肝癌死亡率高发区。我们对从该地区建立的人肝癌裸鼠移植瘤株，（ＨＨＣ＿４、ＨＨＣ＿（１５））细胞ＤＮＡ进行分子生物学研究。应用ＰＣＲ方法，我们证明了ＨＨＣ＿４、ＨＨＣ＿（１５）癌细胞ＤＮＡ都有ＨＢＶＤＮＡ整合。通过ＤＮＡ测序，我们证明了ＨＨＣ＿４ＤＮＡ有ｐ５３基因第２５０密码子的突变；ＨＨＣ＿（１５）有ｐ５３基因第２４９密码子的突变。这为本地区肝癌死亡率与高ＨＢＶ高感率和／或黄曲霉素Ｂ＿１在食品中高污染率的关系提供有力的分子病理学证据。
The Tongan area in Xiamen has become China’s second high mortality area for liver cancer. We conducted molecular biology studies on human hepatocellular carcinoma xenograft tumors (HHC_4, HHC_(15)) cell lines established in this region. Using the PCR method, we demonstrated that HBV DNA was integrated in HHC_4 and HHC_(15) cancer cell DNA. By DNA sequencing, we demonstrated that the HHC_4 DNA has a mutation at codon 250 of the p53 gene; HHC_15 has a mutation at codon 249 of the p53 gene. This provides strong molecular pathological evidence for the relationship between liver cancer mortality and high HBV high-sensitivity and/or high contamination rates of aflatoxin B1 in food for this region.