论文部分内容阅读
肾母细胞瘤过度表达基因（nephroblastomaoverexpressedgene，nov）是法国Perbal等人于1991年发现的一种新的原癌基因，在细胞的生长和调节过程中起重要作用。本研究采用免疫组织化学方法研究了nov基因在大鼠中枢神经系统中的表达。实验共使用5只Wistar大鼠，冰冻切片厚度为20pm。兔抗nov抗血清由法国Perbal教授惠赠。采用敏感的链霉菌生物素一抗生物素免疫组织化学方法（S－ABC）配合微波辅助的抗原修复技术进行染色。结果发现，nov蛋白阳性细胞广泛分布于大脑皮层、海马复合体、丘脑、下丘脑、脑干和脊髓前角等。这些阳性细胞一般是具有2－3个突起的大中型细胞，也有一些未见典型突起的中小型细胞。nov蛋白主要位于胞质和一些突起中。人们已经认识到nov基因的表达产物是一种类胰岛素生长因子结合蛋白，从而认为nov基因属于类胰岛素生长因子家族中的一员，在细胞的生长与分化等调节中起重要作用。nov蛋白在大鼠中枢神经系统中有如此广泛的分布，提示它在中枢神经系统的活动中有重要作用，可能通过类胰岛素生长因子家族对神经系统的发育与分化起一定的调节作用。
Nephroblastoma overexpressed gene (nov) is a new proto-oncogene discovered by Perbal et al. in France in 1991 and plays an important role in cell growth and regulation. In this study, immunohistochemistry was used to study the expression of nov gene in the rat central nervous system. A total of 5 Wistar rats were used in the experiment, and the frozen section thickness was 20 μm. Rabbit anti-nov antiserum received a gift from Professor Perbal of France. Sensitive streptavidin-biotin immunohistochemistry (S-ABC) and microwave-assisted antigen retrieval techniques were used for staining. The results showed that nov protein-positive cells are widely distributed in the cerebral cortex, hippocampal complex, thalamus, hypothalamus, brainstem and anterior horn of the spinal cord. These positive cells are generally large and medium cells with 2-3 protrusions, and also some small and medium cells without typical protrusions. The nov protein is mainly located in the cytoplasm and some processes. It has been recognized that the expression product of the nov gene is an insulin-like growth factor binding protein, so that the nov gene is considered to be a member of the insulin-like growth factor family and plays an important role in the regulation of cell growth and differentiation. The fact that nov protein is widely distributed in the central nervous system of rats suggests that it plays an important role in the activity of the central nervous system and may regulate the development and differentiation of the nervous system through the insulin-like growth factor family.