论文部分内容阅读
收集肝癌等５种消化系癌症患者血清，用免疫电泳法测定血清α１－抗胰蛋酶（α１－ＡＴ）水平，结果发现各癌症组血清α１－ＡＴ水平均显著高于对照组（Ｐ＜０．００１）。另选择培养两种分化不同的消化系胃癌细胞，分离胞浆及胞核ＫＣｌ抽提液，用ＤＣＣ法测定相应性激素受体，结果ＳＧＣ－７９０１细胞胞浆及胞核抽提液中ＥＲ为３０．２和３５．７，ＰＲ为２０．３和２２．７ｆｍｏｌ／ｍｇ蛋白；ＮＫＭ－４５细胞相应ＥＲ为２４．８和２５．１，而ＰＲ为１６．４和１８．６ｆｍｏｌ／ｍｇ蛋白，证明消化系胃癌细胞为ＥＲ、ＰＲ阳性，而分化低恶性高转移强的ＳＧＣ－７９０１细胞ＥＲ、ＰＲ含量较高。这对消化系肿瘤的临床诊断有一定价值，也为其内分泌治疗提供证据。
The serum of 5 digestive cancer patients, including liver cancer, was collected and serum α1-antitrypsin (α1-AT) levels were measured by immunoelectrophoresis. The results showed that serum α1-AT levels in each cancer group were significantly higher than those in the control group (P<0). .001). In addition, two differently differentiated gastric cancer cells of the digestive system were selected and the cytoplasm and nucleus KCl extracts were separated, and the corresponding sex hormone receptors were measured by DCC method. The results showed that the ER in cytosol and nucleus extracts of SGC-7901 cells was 30. .2 and 35.7, PR was 20.3 and 22.7 fmol/mg protein; NKM-45 cells corresponded to ER 24.8 and 25.1, while PR was 16.4 and 18.6 fmol/mg protein, proving Gastric cancer cells in the digestive system were positive for ER and PR, while those in the low-malignant, high-metastatic SGC-7901 cells had higher levels of ER and PR. This has some value in the clinical diagnosis of digestive system tumors and also provides evidence for its endocrine therapy.