论文部分内容阅读
目的:通过去卵巢手术制备小鼠骨质疏松模型,对比研究上、下坡跑台运动对去卵巢小鼠破骨细胞分化的影响,以及NF-κB信号通路在介导运动对破骨细胞分化影响过程中的作用。方法:32只8周龄C57 BL/6雌性小鼠被随机分为4组:假手术安静组(S组)、去卵巢安静组(O组)、去卵巢上坡跑组(U组)和去卵巢下坡跑组(D组)。U组和D组小鼠于摘除卵巢一周后分别进行为期8周的上坡跑和下坡跑训练,每周训练5次,每次训练40 min,跑台坡度分别为±9°,跑速均为0.8 km/h。S组和O组在笼中正常饲养,不进行任何训练。8周后对股骨组织进行抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)染色检测,并对其骨髓进行破骨细胞原代培养,检测破骨细胞分化过程中NF-κB信号通路相关蛋白的表达。结果:各组小鼠股骨TRAP阳性染色区域的平均光密度值和平均阳性染色面积百分比存在显著差异,表现为O组显著高于S组,两运动组显著低于O组,且D组显著低于U组。各组小鼠破骨细胞前体IκBα和p65蛋白相对表达量无显著性差异;而p-IκBα和p-p65蛋白相对表达量组间差异显著,表现为O组显著高于S组,两运动组显著低于O组,且D组显著低于U组。结论:小鼠去卵巢后骨组织破骨细胞特异性酶TRAP大量生成,显示去卵巢手术可引起小鼠骨组织破骨细胞的大量分化和活化,而跑台运动可通过抑制破骨细胞分化过程中p65和IκBα蛋白的磷酸化而有效抑制破骨细胞分化NF-κB信号通路,从而有效抑制骨吸收,且下坡跑效果优于上坡跑。
OBJECTIVE: To study the osteoporosis model of ovariectomized mice by ovariectomy and to compare the effects of treadmill exercise on the differentiation of osteoclasts in ovariectomized mice and the effect of NF-κB signaling pathway on osteoclast differentiation Affect the role of the process. Methods: Thirty-two 32-week-old C57BL / 6 female mice were randomly divided into 4 groups: sham operation group (S group), ovariectomized group (O group), ovariectomized group (U group) Ovariectomy group (D group). After one week of ovariectomy, the U and D mice were trained for 8 weeks of uphill and downhill training respectively, 5 times a week, each training 40 minutes, Both are 0.8 km / h. S and O groups were normally housed in cages without any training. After 8 weeks, the tartrate-resistant acid phosphatase (TRAP) staining was performed on the femur and osteoblast primary culture was performed on the bone marrow to detect the expression of NF-κB signaling pathway-related proteins expression. Results: There was a significant difference between the average optical density value and the average percentage of positive staining area in TRAP positive staining area of femur in each group, which was significantly higher in group O than in group S, significantly lower in group two than in group O, and significantly lower in group D In the U group. The relative expression of IκBα and p65 protein in osteoclast precursors of mice in each group showed no significant difference, while the expression of p-IκBα and p-p65 protein had significant difference, which was significantly higher in group O than in group S Group was significantly lower than the O group, and D group was significantly lower than the U group. CONCLUSION: TRAP, a specific osteoclast-specific enzyme in bone tissue of ovariectomized mice, is abundantly produced, which shows that ovariectomy can induce a large number of osteoclast differentiation and activation in mouse bone tissue. Treadmill exercise can inhibit osteoclast differentiation In p65 and IκBα protein phosphorylation and effectively inhibit osteoclast differentiation NF-κB signaling pathway, which effectively inhibit bone resorption, and downhill run better than uphill run.