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目的：探讨人胃癌ＤＮＡ甲基化情况及检测方法。方法：以甲基化酶温育与３Ｈ－ＳＡＭ掺入法检测２１例人胃癌癌旁和外周正常区域的总基因组ＤＮＡ甲基化水平，并与相同标本的ＨｐａⅡ／ＭｓｐⅠ酶解、Ｓｏｕｔｈｅｒｎｂｏｌｔ分析癌基因甲基化的结果相比较。结果：发现前者检测癌细胞甲基化的敏感性高于后者；而对于癌前病变区，前者的阳性率又低于后者；两种方法都显示癌细胞总基因组ＤＮＡ甲基化和ｃ－ｍｙｃ，ｃ－Ｈａ－ｒａｓ癌基因甲基化水平降低。结论：在人胃癌发生中有ＤＮＡ甲基化的改变，两种检测ＤＮＡ甲基化方法的结果有所不同，可相互借鉴和补充。
Objective: To investigate the DNA methylation status and detection methods of human gastric cancer. METHODS: Methylation and 3H-SAM incorporation were used to detect the total genomic DNA methylation levels in the adjacent and peripheral normal areas of 21 cases of human gastric cancer, and compared with HpaII/MspI digestion and Southernbolt analysis of the same specimens. The results of gene methylation were compared. RESULTS: It was found that the former was more sensitive than the latter in detecting methylation of cancer cells, while the former was less positive in precancerous lesions; both methods showed total DNA methylation in cancer cells and c - Myc, c-Ha-ras oncogene methylation levels decreased. Conclusion: There are changes in DNA methylation in the development of human gastric cancer. The results of the two methods for detecting DNA methylation are different and can be used for reference and supplementation.