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目的和方法：采用两种体外细胞趋化游走实验体系及间接免疫荧光等方法，观察丹参单体（IH764－3）对rhIL－8诱导的人嗜中性白细胞（neutrophils，Neu）跨聚碳酸酯（PC）膜和人脐静脉内皮细胞（humanumbilicalveinendothelialcells，HUVEC）单层趋化游走过程的影响，以分析IH764－3的抗炎作用机制。结果：与对照组比较、IH764－3对rhIL－8诱导的Neu跨PC膜趋化游走（P＜0．01）和跨HUVEC单层趋化游走（P＜0．01）均有明显地抑制作用。这种抑制作用是IH764－3抗炎作用机制之一，与对照组比较、IH764－3对rhIL－8诱导NeuCD11b表达（P＜0．01）及Neu与HUVEC单层粘附（P＜0．01）均有明显抑制作用。结论：这种抑制作用是IH764－3抑制Neu趋化游走的深层机制。
To investigate the effect of Salvia miltiorrhiza monomer (IH764-3) on rhIL-8-induced neutrophils (Neu) trans-polycarbonic acid (PC) membrane and human umbilical vein endothelial cells (HUVEC) monolayers to analyze the anti-inflammatory mechanism of IH764-3. Results: Compared with the control group, IH764-3 had a significant effect on the translocation of Neu across PC membrane (P <0.01) and the trans-HUVEC monolayer (P <0.01) induced by rhIL-8 To inhibit the role. IH764-3 induced the expression of NeuCD11b by rhIL-8 (P <0.01) and the adhesion of Neu with HUVEC monolayer (P <0. 01) have significant inhibitory effect. Conclusion: This inhibitory effect is a profound mechanism by which IH764-3 inhibits Neu chemotaxis.