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国内外研究已证实：粪便某废弃物中的肠道病毒经过厌氧处理后能被灭活，并认为温度和分子态氨是沼气发酵过程中杀灭肠道病毒的主要理化因素。本研究目的是探索沼气发酵料液中，其它微生物（主要是细菌）在病毒灭活中所起的作用。本实验以Ｐｏｌｉｏｌ型病毒为测试病毒，通过用不同的方法处理沼气料液来观察不同时间内病毒的存活情况。实验结报表明：在沼气料液中存在着一种既能为高速离心所沉淀，又能被高温作用后丧失其作用，还能在肉汤中２６℃培养增强其灭病毒活性的物质，即细菌。并认为细菌数量多少对病毒灭活影响不大。
Domestic and foreign studies have confirmed that: Enteric viruses in a waste of manure can be inactivated after anaerobic treatment, and temperature and molecular ammonia are the main physical and chemical factors that kill enterovirus in biogas fermentation process. The purpose of this study was to explore the role of other microorganisms (mainly bacteria) in virus inactivation in biogas fermentation feedstocks. In this experiment, Poliol-type virus was used as the test virus to observe the virus’s survival in different time by using different methods to treat biogas feed liquid. Experimental results show that: there is a biogas feed liquid both for high-speed centrifugal precipitation, but also after the loss of its role of high temperature, but also in the broth at 26 ℃ to enhance their anti-virus activity of the material, that is bacterial. And that the number of bacteria has little effect on virus inactivation.