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为研究胰岛素样生长因子 I(IGF I)反义寡核苷酸转染对人肝癌细胞增生、分化及凋亡的影响 ,探讨寡核苷酸转染治疗肝癌的可行性 ,利用反义核酸技术 ,合成针对IGF I的寡核苷酸片段 ,利用脂质体包裹反义IGF I寡核苷酸片段瞬时转染人肝癌细胞系BEL 74 0 2细胞 ,MTT法检测细胞增生 ;放免法检测培养细胞上清中AFP、CEA的分泌量 ;采用末端标记 (Tunel)法检测细胞凋亡的变化。结果发现转染反义IGF I寡核苷酸可使人肝癌细胞系BEL 74 0 2细胞增生下降 ,AFP、CEA表达降低 ,凋亡细胞数量增多。反义IGF I寡核苷酸转染人肝癌细胞系BEL 74 0 2细胞可以降低细胞增生、减少细胞去分化并诱导肝癌细胞凋亡
To investigate the effect of IGF I antisense oligonucleotide transfection on the proliferation, differentiation and apoptosis of human hepatocellular carcinoma cells, the feasibility of oligonucleotide transfection in the treatment of liver cancer was explored. Antisense oligonucleotides , The oligonucleotide fragment targeting IGF I was synthesized and the human hepatocellular carcinoma cell line BEL 74 0 2 cells were transiently transfected with the antisense IGF I oligonucleotide fragment by liposome and the cell proliferation was detected by MTT assay; The amount of AFP and CEA in the supernatant was measured. The apoptosis was detected by Tunel method. The results showed that transfection of antisense IGF I oligodeoxynucleotides reduced the proliferation of human hepatocellular carcinoma cell line BEL 74 0 2, decreased the expression of AFP and CEA, and increased the number of apoptotic cells. Transfection of human hepatocellular carcinoma cell line BEL 74 0 2 with antisense IGF I oligonucleotides reduced cell proliferation, decreased cell dedifferentiation, and induced apoptosis in hepatocellular carcinoma cells