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以加工番茄品种“石红305”、“里格尔87-5”以及“LA2711”为试材,采用半定量RT-PCR法初步检测了不同浓度盐胁迫下加工番茄叶片中番茄红素β?环化酶(Lyc-β)基因的表达水平,以期探索盐胁迫对加工番茄类胡萝卜素代谢影响的分子机理。结果表明:一定范围的盐浓度可以上调或下调Lyc-β基因的表达,且随着盐浓度的增加,不同加工番茄品种叶片中Lyc-β基因的表达量呈现不均一变化,这可能与品种的基因型以及耐盐性有关;利用HPLC方法对相应叶片中β?胡萝卜素含量进行测定后发现,Lyc-β基因的表达与β?胡萝卜素含量的变化相符,这也在一定程度上说明盐胁迫可通过调控Lyc-β基因的表达量来影响β?胡萝卜素的代谢。
Tomato varieties “Shihong 305 ”, “Ringer 87-5 ” and “LA2711 ” were used as materials, the semi-quantitative RT-PCR method was used to test the effects of different concentrations of salt on tomato leaves In order to explore the molecular mechanism of salt stress on the metabolism of carotenoids in processed tomato. The results showed that a certain range of salt concentration could up-regulate or down-regulate the expression of Lyc-β gene. With the increase of salt concentration, the expression of Lyc-β gene in leaves of different tomato varieties showed a non-uniform change, Genotype and salt tolerance. The content of β-carotenoid in leaves was determined by HPLC method. It was found that the expression of Lyc-β gene was consistent with the change of β-carotenoid content, which also explained to some extent the effect of salt stress Β-carotene metabolism can be influenced by regulating the expression of Lyc-β gene.