人3型副流感病毒（HPIV-3）JS株在20℃适应后制成候选活疫苗,这些不同代次的候选疫苗（cp-12,cp-18和cp-45）具有冷适应（ca）和温度敏感（ts）的特性,均在仓鼠和3型副流感病毒（PIV-3）血清阴性的恒河猴中显示减毒,cp-12和cp-45疫苗还在PIV-3血清阴性的黑猩猩中显示减毒。这些动物模型研究表明,减毒水平和表型稳定性与冷传代 Candidate live vaccines were made at a temperature of 20 ° C by JSIV strains of human parainfluenza virus type 3 (HPIV-3) and had cold acclimation (ca) for these different generations of candidate vaccines (cp-12, cp-18 and cp- And temperature-sensitive (ts) were shown to be attenuated in hamsters and seropositive rhesus monkeys with type 3 parainfluenza virus (PIV-3), and cp-12 and cp-45 vaccines were also serologically negative in PIV-3 seronegative Chimpanzee shows attenuated. Studies in these animal models have demonstrated attenuated levels and phenotypic stability with cold passage