To determine 13 flavonoids and glycyrrhizic acid in licorice(Glycyrrhiza spp.), several samples from different areas were examined by HPLC-DAD analysisT he analysis was performed on a Zorbax Extend-C18(250 mm × 4.6 mm, 5 μm) column connected with a Zorbax Extend guard column(20 mm × 4.6 mm, 5 μm). The mobile phase consisted of(A) acetonitrile and(B) 0.026% aqueous H3PO4(V/V) using a gradient elution of 20%-25% A at 0-20 min, 25%-33% A at 20-30 min, 33%-50% A at 30-55 min, 50%-60% A at 55-65 min, and 60% A between 65 min and 80 min, and peaks were detected at 280 nm. The fourteen compounds were assigned by HPLC-Orbitrap MS methods. The regression coefficient for the linear equations for the 14 compounds ranged between 0.9998 and 1. The limits of detection and quantification lay in the range of 0.032-2.461 and 0.154-8.202 μg·mL-1, respectively. The relative recovery rates for the 14 compounds were in the range of 93.90%-106.73% with RSDs being less than 5%. Coefficient variations for intra-day and inter-day precisions were in the range of 0.27%-2.38% and 0.31%-3.51%, respectively. In summary, the validated method was applied to the simultaneous determination of the 14 components in 29 different licorice samples and was proven to be suitable for quality evaluation of licorices and their active fractions. To determine 13 flavonoids and glycyrrhizic acid in licorice (Glycyrrhiza spp.), Several samples from different areas were examined by HPLC-DAD analysisT he analysis was performed on a Zorbax Extend-C18 (250 mm × 4.6 mm, 5 μm) column connected with a mobile phase consisted of (A) acetonitrile and (B) 0.026% aqueous H3PO4 (V / V) using a gradient elution of 20% -25% A at 0-20 min, 25% -33% A at 20-30 min, 33% -50% A at 30-55 min, 50% -60% A at 55-65 min, and 60% A between 65 min and 80 min, and peaks were detected at 280 nm. The fourteen compounds were assigned by HPLC-Orbitrap MS methods. The regression coefficient for the linear equations for the 14 compounds ranged between 0.9998 and 1. The limits of detection and quantification lay in the range of 0.032-2.461 and 0.154-8.202 μg · mL-1, respectively. The relative recovery rates for the 14 compounds were in the range of 93.90% -106.73% with RSDs being less than 5%. Coefficient variations f or intra-day and inter-day precisions were in the range of 0.27% -2.38% and 0.31% -3.51%, respectively. In summary, the validated method was applied to the simultaneous determination of the 14 components in 29 different licorice samples and was proven to be suitable for quality evaluation of licorices and their active fractions.