骨切片的染色法有多种,其中硫堇 苦味酸染色法是比较常用的染色方法,但书中及各实验室的染液配制及染色方法不尽相同[1～4] ,因此我们对2种硫堇染液和2种脱水、透明方法做了一些比较和探讨,以便选择一种效果较好的染色方法。1　材料和方法采用经1 0 %甲醛固定的人长骨,锯成3mm Bone slices of a variety of staining methods, of which thionine picric acid staining method is more commonly used staining method, but the book and the laboratory preparation and dyeing method dye is not the same [1 ~ 4], so we 2 Thionine dye solution and two kinds of dehydration, transparent method to do some comparison and discussion in order to choose a better staining method. 1 Materials and Methods The human long bone fixed with 10% formaldehyde, sawing into 3mm