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本文报导雷公藤T_Ⅱ(由江苏皮肤病防治研究所提取)与黄精提取物 P_0(本院临床医学实验科药理实验室提取)在体外试验中,对T淋巴细胞的作用,初步发现,雷公藤T_Ⅱ具有抑制T细胞转化和形成自然花环细胞(ERFC)的能力;黄精P_0则可使部分细胞免疫功能低下的病人,其淋巴细胞的转化率和ERFC百分率提高。方法: ~3H-胸腺嘧啶核苷(~H-TdR)掺入淋巴细胞转化试验: 基本上参照我室过去所用方法。所用培养基为RPMI 1640培基,加入20％小牛血清及谷胺酰胺100毫克％,分装每管3毫升,加入1％PHA(自制)0.2毫升,健康人或病人肝素抗凝血0.2毫升。每份标本同时接种6管
This article reports on the effects of Triptoid T_II (extracted by the Institute of Dermatology and Prevention of Jiangsu) and Huangjing extract P_0 (Extracted from the Pharmacological Laboratory of Clinical Medical Laboratory of this hospital). In vitro experiments, the role of T lymphocytes, preliminary findings, triptolide T_II Has the ability to inhibit T cell transformation and the formation of natural rosette cells (ERFC); Huang Jing P_0 can make some patients with low cellular immune function, the lymphocyte conversion rate and ERFC percentage increase. METHODS: ~3H-thymidine (~H-TdR) incorporation into lymphocyte transformation assays: Basically, the method used in our laboratory has been used in the past. The culture medium used was RPMI 1640, and 20% calf serum and glutamine 100 mg% were added, 3 ml per tube was dispensed, 0.2 ml of 1% PHA (homemade) was added, and anticoagulation 0.2 ml of heparin was administered to healthy persons or patients. . Simultaneous inoculation of 6 tubes per specimen