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微生物是土壤环境变化的敏感因子,为探明再生水灌溉和氮素减量施用对土壤环境的影响,以温室棚栽番茄为研究对象,借助Miseq高通量技术,比较研究了再生水灌溉下氮素常规施肥和氮肥减量施用对土壤细菌群落结构所产生的影响,并采用冗余分析(Redundancy Analysis,RDA)方法分析导致土壤细菌群落结构差异的因素.结果表明:再生水灌溉对土壤硝化螺菌门(Nitrospirae)、芽单胞菌门(Gemmatimonadetes)、厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)和放线菌门(Actinobacteria)群落结构的影响明显;再生水灌溉土壤细菌共53个属,其中41个属是再生水灌溉和清水灌溉土壤的共有菌属,其余12个属是再生水灌溉土壤的特有菌属.随氮素施用水平的降低,土壤细菌种群优势度呈先增加后降低然后再增加的趋势,减少氮肥施用有利于土壤细菌种群丰富度和多样性的增加.土壤微生物群落结构受土壤化学特性的影响.再生水灌溉能够促进与土壤碳、氮转化相关的微生物的增长,改变土壤微生物的群落结构.
Microorganism is a sensitive factor of soil environmental changes. To study the effect of reclaimed water irrigation and nitrogen application on soil environment, greenhouse greenhouse planted tomatoes were used as research objects. Miseq high-throughput technique was used to study the effects of nitrogen The effects of conventional fertilization and nitrogen fertilizer application on soil bacterial community structure were analyzed, and the factors that led to the differences in soil bacterial community structure were analyzed by the method of Redundancy Analysis (RDA). The results showed that: Nitrospirae, Gemmatimonadetes, Firmicutes, Proteobacteria and Actinobacteria communities were significantly affected. There were altogether 53 genera in the soil of reclaimed water irrigated soil, Among them, 41 genera were common fungi of reclaimed water irrigation and fresh water irrigated soil, and the remaining 12 genera were endemic genera of reclaimed water irrigated soil. With the decrease of nitrogen application level, the dominant bacterial population dominance increased first and then decreased and then increased The trend of reducing nitrogen fertilizer application is beneficial to the increase of soil bacterial population richness and diversity.The soil microbial community structure is affected by soil Effect pharmacodynamic properties. Reclaimed water for irrigation can promote growth of microorganisms associated with soil carbon, nitrogen transformation, soil microbial community structure changes.