论文部分内容阅读
Objective: To observe the effect and mechanism of Xiangsha Liujunzi decoction (XSLJZD) drug serum on gastric antrum smooth muscle cells (SMCs) in rats with functional dyspepsia (FD).Methods: Gastric antrum SMCs from rats with FD were isolated,cultured,and then divided into six groups as follows: control,model,domperidone,low-dose XSLJZD (LXSLJZD),medium-dose XSLJZD (MXSLJZD),and high-dose XSLJZD (HXSLJZD).Each group was administered the corresponding drug serum for intervention.Drug serum intervention conditions and proliferative activity of SMCs were tested by cholecystokinin octapeptide.Ghrelin,gastrin,somatostatin,and substance P (SP) levels were measured by ELISA.Somatostatin and SP mRNA expression was measured by real-time PCR.Results: A concentration of 10％ drug serum for 24 h was decided to be the best intervention condition for later study.The mean optical density value in the model group was lower than that in the control group (P =.001).Optical density values in the domperidone and HXSLJZD groups were higher than those in the model group (P =.025,P =.032,respectively).Gastrin,SP,and ghrelin levels in the model group were lower (P =.007,P =.037,P =.005,respectively),but somatostatin levels were higher,compared with those in the control group (P =.031).Gastrin,SP,and ghrelin levels in the domperidone,MXSLJZD,and HXSLJZD groups were higher than those in the model group (all P＜.05).Somatostatin levels in the four drug-treated groups were lower than those in the model group (P =.002,P =.007,P =.001,P =.009,respectively).SP mRNA levels in the model group were lower than those in the control,domperidone,MXSLJZD,and HXSLJZD groups (P =.037 P =.016,P =.025,P =.002,respectively).Somatostatin mRNA levels in the model group were higher than those in the control and MXSLJZD groups (P =.042,P =.035).Conclusions: XSLJZD and domperidone drug serum effectively promote proliferative activity of gastric antrum SMCs in an FD model.The mechanism of this activity may be regulated by gastrointestinal hormones.