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目的:探讨胆管癌组织中血管内皮生长因子C(VEGF-C)的表达及微淋巴管密度(MLVD)与胆管癌临床病理特征及预后的关系。方法:应用免疫组化法分别检测47例胆管癌、40例近癌的非癌胆管及15例正常肝外胆管组织中VEGF-C的表达和D2-40标记的MLVD。分析两者间的相关性,及其与临床病理特征和预后的关系。结果:胆管癌组织中VEGF-C的阳性表达率明显高于近癌的非癌胆管和正常胆管组织(均P<0.05),胆管癌和近癌的非癌胆管组织中MLVD明显高于正常胆管组织(均P<0.05);VEGF-C的表达以及MLVD与胆管癌浸润深度、TNM分期、淋巴结转移密切相关(P<0.05),且MLVD还与胆管癌分化程度有关(P<0.05);VEGF-C阳性胆管癌组织MLVD明显大于其阴性胆管癌组织(P<0.05),且VEFG-C表达与MLVD呈正相关(r=0.615,P<0.05);VEFG-C阴性表达患者预后明显优于其阳性表达患者,复发者胆管癌组织中的MLVD明显高于无复发者(P<0.05),且MLVD与患者生存期呈负相关(r=-0.542,P<0.05)。结论:VEGF-C的表达和MLVD与管癌淋巴结转移、预后密切相关,VEGF-C可能是预测胆管癌淋巴结转移的有效指标之一。
Objective: To investigate the relationship between the expression of vascular endothelial growth factor C (VEGF-C) and the lymphatic vessel density (MLVD) in cholangiocarcinoma and the clinicopathological features and prognosis of cholangiocarcinoma. Methods: The expressions of VEGF-C and D2-40-labeled MLVD in 47 cases of cholangiocarcinoma, 40 cases of non-cancerous bile duct and 15 cases of normal extrahepatic bile duct were detected by immunohistochemistry. Analyze the correlation between the two, and its relationship with clinicopathological features and prognosis. Results: The positive expression rate of VEGF-C in cholangiocarcinoma was significantly higher than that in non-cancerous cholangiocarcinoma and normal cholangiocarcinoma (all P <0.05). The MLVD in cholangiocarcinoma and non-cancerous cholangiocarcinoma was significantly higher than that in normal cholangiocarcinoma (P <0.05). The expression of VEGF-C and MLVD were closely related to the depth of invasion, TNM stage and lymph node metastasis (P <0.05), and the expression of VEGF was related to the differentiation of cholangiocarcinoma (P <0.05) The positive rate of MLVD in C-positive cholangiocarcinoma was significantly higher than that in negative cholangiocarcinoma (P <0.05), and the expression of VEFG-C was positively correlated with MLVD (r = 0.615, P <0.05) The positive expression of MLVD in patients with recurrent cholangiocarcinoma was significantly higher than that without recurrence (P <0.05), and the MLVD was negatively correlated with the survival of patients (r = -0.542, P <0.05). Conclusion: The expression of VEGF-C and MLVD are closely related to lymph node metastasis and prognosis of carcinoma, and VEGF-C may be one of the effective indexes for predicting lymph node metastasis of cholangiocarcinoma.