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引进陆地棉(Gossypium hirsutum)种质材料的性状-标记关联分析是发掘其优异基因并在分子标记辅助育种中使用的科学依据。本研究利用199个SSR标记和42个In Del标记对94份国外陆地棉种质材料和27份新疆本地品种的纤维品质和农艺学性状进行全基因组关联分析。遗传结构和Neighbor-Join聚类分析将陆地棉种质群体划分为2个亚群,连锁不平衡分析发现有9.42%的位点组合存在LD(p<0.01,r2≥0.05);基于混合线性模型的关联分析结果显示,能够同时在2个播期检测到的与6个纤维品质性状、4个产量性状和4个农艺性状显著关联的标记位点分别有18个、10个和5个,平均表型变异解释率分别为6.06%(1.17%~13.86%)、4.62%(1.18%~15.28%)和6.75%(1.87%~9.98%)。上述关联位点中共检测到188个等位变异,其中表型效应值大于0.6的优异等位变异有12个。研究结果可进一步用于分子辅助育种,并为基因精细定位提供参考资料。
The trait-marker association analysis of germplasm introduced from Gossypium hirsutum is the scientific basis for discovering its excellent gene and using it in molecular marker-assisted breeding. In this study, 199 SSR markers and 42 In Del markers were used for genome-wide association analysis on the fiber quality and agronomic traits of 94 accessions from upland cotton from China and 27 from Xinjiang. Genetic structure and Neighbor-Join clustering analysis divided upland cotton germplasms into two subgroups. Linkage disequilibrium analysis showed that 9.42% loci had LD (p <0.01, r2≥0.05). Based on the mixed linear model The results of correlation analysis showed that 18, 10 and 5 marker sites that could be significantly correlated with 6 fiber quality traits, 4 yield traits and 4 agronomic traits simultaneously detected at 2 sowing dates were averaged The explanatory rates of phenotypic variation were 6.06% (1.17% ~ 13.86%), 4.62% (1.18% ~ 15.28%) and 6.75% (1.87% ~ 9.98%), respectively. A total of 188 alleles were detected in the above-mentioned association sites, of which 12 were excellent alleles with phenotypic effects greater than 0.6. The results can be further used for molecular assisted breeding and provide references for fine mapping of genes.