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目的对北京市2012年-2015年腹泻患者粪便标本中类志贺邻单胞菌进行荧光定量PCR检测并了解其耐药情况。方法对收集的31株类志贺邻单胞菌进行传统生化鉴定后,用荧光定量PCR对菌株进行23S rRNA基因和志贺菌侵袭性质粒H抗原基因(ipa H)检测,用血清凝集试验检测是否与志贺菌有交叉凝集,用微量肉汤稀释法进行14种药物敏感试验。结果传统方法鉴定的31株类志贺邻单胞菌,经荧光定量PCR检测只有28株为类志贺邻单胞菌且ipa H均为阴性,与志贺菌属诊断血清交叉凝集率为7.14%;28株类志贺邻单胞菌对亚胺培南100%敏感,对阿莫西林/克拉维酸耐药率最高,为92.9%,且有24株菌在最高稀释度仍生长;出现了7种耐药型别和13种耐药谱,耐3种以上药物的耐药率为75.0%。结论类志贺邻单胞菌检测时可把生化和荧光定量PCR结合进行;类志贺邻单胞不携带ipa H,存在多重耐药现象,临床治疗可首选亚胺培南。
OBJECTIVE: To detect the phenotypes of Pseudomonas solania in stool samples of diarrhea patients in Beijing from 2012 to 2015 by fluorescence quantitative PCR and to understand its drug resistance. Methods After the traditional biochemical identification of 31 strains of isolated strains of Shigella spp., The 23S rRNA gene and the ipa H gene of Shigella spp. Plasmids were detected by fluorescence quantitative PCR. The serum agglutination test was used to determine whether With Shigella cross-coagulation, with a small amount of broth dilution method for 14 kinds of drug sensitivity test. Results Of the 31 strains identified by the traditional method, only 25 strains were identified as Pseudomonas solani by fluorescence quantitative PCR, and all the isolates were negative with ipa H. The cross-coagulation rate with Shigella serogroups was 7.14 %; 28 strains of Shigella spp. Were 100% sensitive to imipenem, the highest resistance rate to amoxicillin / clavulanic acid was 92.9%, and 24 strains were still growing at the highest dilution; Seven kinds of resistance patterns and 13 kinds of resistance spectrum were tested, and the resistance rate of three or more kinds of drugs was 75.0%. Conclusion The detection of Shigella spp. By biochemical and real-time PCR can be carried out. The ipa H-like cells do not carry ipa H and have multiple drug resistance. Imipenem is the first choice for clinical treatment.