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目的探讨敛肝熄风养血濡筋方对帕金森病(Parkinson’s disease,PD)大鼠黑质抗凋亡蛋白-2(Bcl-2)、促凋亡蛋白(Bax)表达的影响。方法采用6-羟基多巴胺(6-OHDA)纹状体左侧两点注射法复制PD大鼠模型,术后将大鼠随机分为空白对照组、模型组、美多巴组(0.075 g·kg-1)、敛肝熄风养血濡筋方(LXYR)(32,16,8g·kg-1)剂量组,连续灌胃4周。实验结束后,免疫组化法检测各组大鼠黑质组织Bcl-2和Bax蛋白的表达。结果与空白对照组比较,模型组Bcl-2和Bax蛋白表达增加(均P<0.01)。与模型组比较,敛肝熄风养血濡筋方高、中剂量组均能增加Bcl-2蛋白表达,降低Bax蛋白表达,差异有统计学意义(P<0.05),其中以高剂量组改变最为显著(P<0.01),而低剂量组及美多巴组与模型组比较差异无统计学意义(P跃0.05)。结论敛肝熄风养血濡筋方可通过调节Bcl-2/Bax表达数量而起到保护黑质多巴胺能神经元的作用。
Objective To investigate the effect of Radix Astragali, nourishing and replenishing blood, nourishing blood and reinforcing tendon recipe on the expression of Bcl-2 and Bax in substantia nigra of Parkinson’s disease (PD) rats. Methods PD rats were made by injection of 6-hydroxydopamine (6-OHDA) into the left side of the striatum. The rats were randomly divided into blank control group, model group, and metoprolol group (0.075 g · kg -1) -1), and the dose of LXYR (32, 16, and 8 g · kg -1) was given to the rats for 4 weeks. At the end of the experiment, the expression of Bcl-2 and Bax protein in substantia nigra of rats in each group was detected by immunohistochemistry. Results Compared with the blank control group, the expression of Bcl-2 and Bax in model group increased (all P <0.01). Compared with the model group, Bcl-2 protein expression and Bax protein expression were both increased (P <0.05), especially in the high-dose group (P <0.01), while there was no significant difference between low dose group and model group (P> 0.05). CONCLUSION Convergence of Liver Qi Xunfu nourishing tendon can protect the nigral dopaminergic neurons by regulating the expression of Bcl-2 / Bax.