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以ＭＳ液体培养基培养２０ｄ左右至菌丝大量生长并紧密交织成团时病菌即可大量产毒，病菌是否产孢对产毒量大小无显著影响。毒素原液中仅非蛋白部分具致病活性，经高温高压灭菌１５ｍ ｉｎ 后活性仍不丧失，且有所加强。该毒素经冷冻或灭菌后于室温下可保存２个月以上而不失活。毒素粗提液中活性成分为一类极性较大的物质，易溶于水、甲醇、乙醇，可溶于正丁醇，微溶于乙酸乙酯，不溶于苯，可被活性炭吸附并为甲醇所洗脱。试验表明，毒素粗提液的ｐＨ值和渗透势分别为６．０和１．２２巴，毒素原液的毒性非ｐＨ值或渗透势所致，从而确定了病菌人工培养滤液中存在有具致病活性的毒素物质。
MS liquid culture medium for about 20d to mycelial growth and closely interwoven into a mass of bacteria can produce a large number of drugs, bacteria whether sporulation on the size of the virus had no significant impact. Only the non-protein part of the toxin stock solution has pathogenic activity, and the activity is still not lost after being sterilized by autoclaving for 15 minutes, and has been strengthened. The toxin can be stored at room temperature for more than 2 months without deactivation after being frozen or sterilized. The crude extract of toxins active ingredients for a class of more polar substances, soluble in water, methanol, ethanol, soluble in n-butanol, slightly soluble in ethyl acetate, insoluble in benzene, can be activated carbon adsorption and Methanol eluted. The results showed that the pH value and osmotic potential of the crude toxin extract were 6.0 and 1.22 bar, respectively. The toxicity of the toxin stock solution was caused by the non-pH or osmotic potential. Thus, it was confirmed that there was pathogenicity in the artificial culture filtrate Active toxins.