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目的:通过传统Ames试验与改进彷徨试验对槲皮素的致突变试验结果,比较两种试验的灵敏度。方法:Ames试验方法采用平板掺入法,选择3.2、16、80、400、2000μg/mL 5个剂量组的槲皮素在有或无代谢话化条件下处理鼠伤寒沙门氏菌株TA98和TA100 48小时后计数回复突变菌落数;改进彷徨试验采用96孔板法,选择0.128、0.64、3.2、16、80μg/mL 5个剂量组的槲皮素在有或无代谢活化条件下处理鼠伤寒沙门氏菌株TA98和TA100 24小时后计数变色孔数。结果:Ames试验结果显示槲皮素在80-2000μg/mL回复突变菌落数明显增加并超过对照2倍并有明显剂量反应关系,Ames试验结果阳性;改进彷徨试验结果显示槲皮素在0.64-80μg/mL变色孔数明显增加(P<0.01),并有剂量反应关系,结果为阳性;而在0.64-16μg/mL剂量下,改进彷徨试验能检测出阳性结果,而Ames试验未检测出阳性结果。结论:在较低浓度时,改进彷徨试验能检测出Ames试验检测不出的阳性结果,表明改进彷徨试验灵敏度较Ames试验高。
OBJECTIVE: To compare the mutagenicity test results of quercetin with the traditional Ames test and the improved imitation test, and to compare the sensitivity of the two tests. Methods: Ames test method using plate incorporation method, the choice of 3.2,16,80,400,2000 g / mL quercetin 5 doses of the group with or without metabolic conversation under the conditions of Salmonella typhimurium TA98 and TA100 48 hours And then count the number of colonies returning to the mutation; improve imitation 徨 test using 96-well plate method, select 0.128,0.64,3.2,16,80μg / mL quercetin 5 doses of the group with or without metabolic activation of Salmonella typhimurium strain TA98 And TA100 Count the number of color change holes 24 hours later. Results: The results of Ames test showed that the number of colonies returning to quercetin at 80-2000μg / mL was significantly higher than that of the control at 2-fold and the dose-response relationship was obvious. The results of Ames test were positive. / mL showed a significant increase (P <0.01) and a dose-response relationship with a positive result. However, at the dose of 0.64-16 μg / mL, positive results could be detected with the improved imitation test, whereas positive results were not detected with the Ames test . CONCLUSIONS: At lower concentrations, the improved imitation test can detect positive results not detected by the Ames test, indicating that the modified imitation test is more sensitive than the Ames test.