论文部分内容阅读
目的:建立胰岛素抵抗大鼠模型,比较胰岛素抵抗及雌激素水平增高对子宫内膜增殖的影响。方法:给予性成熟的Sprague Dawley大鼠高脂饮食以建立胰岛素抵抗大鼠模型,40周后对大鼠行去势手术后,灌胃给予大鼠17β-雌二醇4周。无胰岛素抵抗大鼠为对照组。通过测定葡萄糖耐量、胰岛素耐量、胰岛素抵抗指数、血脂等确定胰岛素抵抗。通过阴道脱落细胞巴氏染色判断雌激素的作用,HE染色检测实验组与对照组大鼠子宫内膜增殖的情况。结果:高脂饮食大鼠的葡萄糖耐量、胰岛素耐量试验曲线下面积及胰岛素抵抗指数均增加。给予雌激素后,阴道脱落细胞巴氏染色可见成熟阴道上皮细胞,HE染色可见子宫内膜厚度明显增加;与无胰岛素抵抗的大鼠相比,有胰岛素抵抗的大鼠子宫内膜出现鳞状细胞化生比例大,子宫间质中嗜酸性粒细胞增多。结论:胰岛素抵抗大鼠发生子宫内膜增殖时更易发生子宫内膜鳞状细胞化生,这一现象可能与体内高胰岛素水平有关。
OBJECTIVE: To establish a rat model of insulin resistance and compare the effects of insulin resistance and estrogen levels on the proliferation of endometrium. Methods: The rats with sexual maturity were given a high-fat diet to establish the model of insulin resistance. After 40 weeks of operation, the rats were administered with 17β-estradiol by gavage for 4 weeks. Rats without insulin resistance as control group. Insulin resistance was determined by measuring glucose tolerance, insulin resistance, insulin resistance index, lipids and the like. Estrogen was evaluated by papillary staining of vaginal cells, HE staining was used to detect endometrial proliferation in experimental group and control group. Results: The area under the curve of glucose tolerance and insulin resistance test and insulin resistance index increased in high fat diet rats. After administration of estrogen, mature vaginal epithelial cells could be seen by Pap test in vaginal exfoliated cells, and the thickness of endometrium was obviously increased by HE staining. Compared with those without insulin resistance, squamous cells appeared in the endometrium of insulin resistant rats A large proportion of metaplasia, uterine interstitial eosinophils increased. Conclusion: Endometrial squamous metaplasia is more likely to occur in endometrial hyperplasia in insulin resistance rats, which may be related to the high insulin level in vivo.