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目的 探讨颞叶癫痫发作大鼠海马TrkB mRNA及其蛋白表达的动态变化特征。方法 建立匹罗卡品(PILO)颞叶癫痢大鼠模型,应用原位杂交及免疫组织化学方法分别检测致(?)大鼠海马齿状回、CA3区及CA1区TrkB nRNA及其蛋白质表达的变化。结果 PILO致(?)后3～6 h,海马齿状回颗粒细胞层、CA1、CA3区锥体细胞层TrkB mRNA表达显著增高(P<0.01),稍后TrkB蛋白表达也随之增高。第7-30 d,TrkB mRNA及其蛋白在齿状回、CA3区呈现第二次表达增强。结论在癫(?)发作早期,TrkB表达增强,提示其可能参与急性癫痫状态的发生;后期表达增强则可能参与了海马的可塑性反应而与慢性自发性发作形成有关。
Objective To investigate the dynamic changes of TrkB mRNA and protein in hippocampus of temporal lobe epilepsy rats. Methods The temporal lobe epilepsy rat models of pilocarpine (PILO) were established. TrkB nRNA and its protein expression in hippocampal dentate gyrus, CA3 and CA1 regions were detected by in situ hybridization and immunohistochemistry respectively The change. Results TrkB mRNA expression in hippocampal dentate gyrus granule cell layer and CA1, CA3 pyramidal cell layer increased significantly (P <0.01) 3 ~ 6 h after PILO induced (?), And later TrkB protein expression also increased. From the 7th day to the 30th day, TrkB mRNA and its protein showed a second increase in the dentate gyrus and CA3 region. Conclusion The increased expression of TrkB in the early stage of epilepsy suggests that it may be involved in the development of acute epilepsy. The increased expression of late TrkB may be involved in the plastic response in the hippocampus, which may be related to the formation of chronic spontaneous seizures.