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目的:探讨母血及脐带血IGF2甲基化状态及其影响因素,评价健康产妇及新生儿脐血IGF2甲基化状态对新生儿出生结局的影响。方法:于2008年4～6月对82名住院产妇进行问卷调查,并采集产妇外周血及其新生儿脐带血,卧式身长计、头围尺分别测量新生儿身高和头围;电子秤称量新生儿体重。RT-PCR分析IGF2 P2、P3启动子区的甲基化状态。结果:新生儿平均身高为49.46 cm,平均体重为3.19 kg,平均头围为33.87 cm。母血IGF2 P2甲基化率的均值是3.00%,脐血是3.81%,脐血IGF2 P2甲基化率高于母血IGF2 P2甲基化率(P<0.01)。母血IGF2 P3甲基化率的均值是5.61%,脐血是4.34%,母血IGF2 P3甲基化率高于脐血IGF2 P3甲基化率(P<0.01)。多元线性回归模型结果显示新生儿IGF2 P2的甲基化状态与母体IGF2 P2的甲基化状态呈正相关(r=0.246,P<0.05),且新生儿IGF2 P3的甲基化状态与母体IGF2 P3的甲基化状态也呈正相关(r=0.428,P<0.01);母体IGF2 P2的甲基化状态与母亲孕期体重增加、被动吸烟史分别相关(分别r=0.234,P<0.05,r=0.242,P<0.05),母体IGF2 P3的甲基化状态还与母亲受教育程度高具有相关性(r=-0.239,P<0.05)。结论:产妇及新生儿脐血IGF2甲基化状态受环境因素影响,但对新生儿出生结局无明显影响。
Objective: To investigate the methylation status of IGF2 in maternal and umbilical cord blood and its influencing factors to evaluate the effect of umbilical blood IGF2 methylation status on neonatal birth outcomes. Methods: From April to June 2008, 82 hospitalized mothers were investigated by questionnaire, and the maternal peripheral blood and cord blood of their newborns were collected. Newborn weight. RT-PCR analysis of IGF2 P2, P3 promoter methylation status. Results: The average neonatal height was 49.46 cm, the average body weight was 3.19 kg, and the average head circumference was 33.87 cm. The mean methylation rate of IGF2 P2 in maternal blood was 3.00%, that in cord blood was 3.81%, and the methylation rate of IGF2 P2 in cord blood was higher than that of maternal blood in IGF2 P2 (P <0.01). The mean methylation rate of IGF2 P3 in maternal blood was 5.61%, that of cord blood was 4.34%, and the methylation rate of IGF2 P3 in maternal blood was higher than that of cord blood IGF2 P3 (P <0.01). Multivariate linear regression model showed that the methylation status of IGF2 P2 in neonates was positively correlated with the methylation status of IGF2 P2 in the newborn (r = 0.246, P <0.05) (R = 0.428, P <0.01). The methylation status of maternal IGF2 P2 was correlated with maternal weight gain and passive smoking history (r = 0.234, P <0.05, r = 0.242 , P <0.05). The methylation status of maternal IGF2 P3 was also correlated with high maternal education level (r = -0.239, P <0.05). Conclusion: The methylation status of IGF2 in umbilical blood of mothers and newborns is affected by environmental factors, but has no significant effect on the neonatal outcome.