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将脂质体包裹的ＩＬ－２基因直接注射至Ｂ１６Ｆ１０黑色素瘤瘤体内，研究肿瘤细胞和肿瘤浸润性淋巴细胞（ＴＩＬ）的功能变化。１０ｄ后，Ｎｏｒｔｈｅｒｎｂｌｏｔ鉴定显示瘤体内注射ＩＬ－２基因后肿瘤细胞及ＴＩＬ中ＩＬ－２ｍＲＮＡ表达阳性；经Ｇ４１８筛选后的肿瘤细胞培养上清中可检测出ＩＬ－２；肿瘤细胞表面表达了较高水平的ＭＨＣⅠ类抗原（Ｈ－２Ｋ￣ｂＤ￣ｂ）；ＴＩＬ表面粘附分子ＬＡＦ－１表达也明显高于对照组，其杀伤Ｂ１６细胞的活性明显增强。结果表明瘤体内注射脂质体包裹的ＩＬ－２基因后，可在肿瘤原位将基因转移至肿瘤细胞及ＴＩＬ中，提高肿瘤细胞的ＭＨＣⅠ类抗原的表达，增强肿瘤细胞的免疫原性，并通过提高ＴＩＬ粘附分子的表达，共同促进ＴＩＬ的肿瘤特异性杀伤功能。
The liposome-encapsulated IL-2 gene was directly injected into the B16F10 melanoma tumor to study the functional changes of tumor cells and tumor infiltrating lymphocytes (TIL). After 10 days, Northern blot identification showed IL-2 gene expression in tumor cells and TIL was positive after intratumoral injection of IL-2 gene; IL-2 was detected in culture supernatant of tumor cells screened by G418; tumor cell surface expressed higher The level of MHC class I antigen (H-2K ̄bD ̄b); TIL surface adhesion molecule LAF-1 expression was also significantly higher than the control group, which significantly enhanced the activity of killing B16 cells. The results showed that intratumoral injection of liposome-encapsulated IL-2 gene can transfer genes to tumor cells and TIL in situ in tumors, increase the expression of MHC class I antigens in tumor cells, and enhance the immunogenicity of tumor cells. By increasing the expression of TIL adhesion molecules, the tumor-specific killing function of TIL is jointly promoted.