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将含IL-6cDNA的PMAM重组载体用脂质体法转染大肠癌细胞株HT-29,克隆出抗性细胞,经Southern印迹杂交证明有IL6基因片段的插入.RNA点杂交结果表明:IL-6基因转染细胞有较强的IL-6mRNA转录,而未转染细胞的转录水平很弱;7TD1-MTT法检测转染前后细胞的上清显示:抗性细胞分泌的IL-6活性明显增强.
The PMMA recombinant vector containing IL-6 cDNA was transfected into colorectal cancer cell line HT-29 by liposome method. The resistant cells were cloned. The insertion of IL6 gene fragment was confirmed by Southern blot hybridization. The result of RNA dot hybridization showed that: 6 The transfected cells had strong IL-6 mRNA transcription, but the transcription level of untransfected cells was weak. The supernatants of cells before and after transfection detected by TD1-MTT assay showed that the activity of IL-6 secreted by resistant cells was significantly increased. .