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血浆Lp(a)浓度增加(>300mg/L)是心血管病的一个特别危险因素,即使胆固醇水平在正常范围,对冠心病的危险性也会增加二至三倍。据研究发现,在441个病例中有194例不能电泳出Lp(a)区带,这种明显的缺点可能与免疫斑点技术的敏感性有关,作者对此方法进行改进提高了敏感性,测定北美人群的正常Lp(a)遗传表型,分析了Lp(a)的等位基因频率。电泳:取标本18μl 与新鲜配制的标本缓冲液(50g/L 的SDS4ml,巯基乙醇800μl,750ml/L 甘油溶液800μl,10g/L 溴酚蓝溶液200μl)100μl 混合,在沸水中加热10分钟,然后取10μl 加入凝胶孔顶部,电3.5%聚胶(pH6.1)和6.5%分离胶中作垂直电
An increase in plasma Lp (a) concentration (> 300 mg / L) is a particularly risk factor for cardiovascular disease, with a 2-3 fold increased risk of coronary heart disease even with normal cholesterol levels. According to the study, Lp (a) bands were not detected in 194 out of 441 cases. This apparent shortcoming may be related to the sensitivity of immunoblotting techniques. The authors improved the method to improve the sensitivity of the assay for North American The normal Lp (a) phenotype of the population was analyzed for the allele frequency of Lp (a). Electrophoresis: Take 18μl of sample and 100μl of freshly prepared sample buffer (SDS4ml of 50g / L, 800μl of mercaptoethanol, 800μl of 750ml / L glycerol solution and 200μl of 10g / L bromophenol blue solution), heat in boiling water for 10 minutes and then Take 10μl added to the top of the gel, electric 3.5% polygel (pH6.1) and 6.5% separation gel for vertical power