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采用改良的CTAB法、高盐低pH法、SDS法、苯酚法等9种提取液提取黄芪干燥根的基因组DNA,结果表明,CTAB法和高盐低pH法能得到高质量的基因组DNA。用这两种方法提取9批黄芪干燥根,A260/A280值均在1.8～2.0之间,琼脂糖凝胶电泳条带清晰,降解较少,能有效的从黄芪干药材中提取到高质量的基因组DNA。将DNA以引物5S进行PCR扩增,获得清晰的条带。本研究从富含多糖和次生物质的黄芪干燥根提取高质量的基因组DNA,为今后黄芪遗传多样性、分子鉴定研究奠定基础。
The genomic DNA of Astragalus mongholicus was extracted by modified CTAB method, high salt and low pH method, SDS method and phenol method. The results showed that genomic DNA was obtained by CTAB method and high salt and low pH method. Using these two methods to extract nine batches of Radix Astragali dry roots, A260 / A280 values were between 1.8 to 2.0, agarose gel electrophoresis band clear, less degradation, can be effectively extracted from Astragalus dry high quality Genomic DNA. The DNA was PCR amplified with primer 5S to obtain a clear band. In this study, high quality genomic DNA was extracted from the dried roots of Radix Astragali which is rich in polysaccharides and secondary metabolites, which laid the foundation for further study on the genetic diversity and molecular identification of Radix Astragali.