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探讨抗凋亡基因ｂｃｌ－２蛋白在常见肝肿瘤发生中的作用。方法应用ｂｃｌ－２癌蛋白单克隆抗体对６４例四种类型的肝恶性肿瘤进行ＬＳＡＢ免疫组织化学标记。结果１６．２％（６／３７）的肝细胞癌，６９．２％（９／１３）的胆管细胞癌，２０．０％（１／５）的肝母细胞瘤和２２．２％（２／９）的肝转移癌呈ｂｃｌ－２癌蛋白阳性表达，其中胆管细胞癌的阳性率显著高于其癌旁组织（Ｐ值＜０．０５）和肝细胞癌（Ｐ值＜０．０１）。癌旁组织中的阳性细胞主要为增生的小胆管上皮细胞，而在肝细胞不典型增生中阳性率颇低（１５．４％，２／１３）。结论ｂｃｌ－２基因可能在胆管细胞癌的发生中起重要作用，而不参与肝细胞的分裂、增殖。
To explore the role of anti-apoptosis gene bcl-2 protein in the development of common liver tumors. Methods LSAB immunohistochemistry was performed on 64 types of hepatic malignancies using monoclonal antibodies against bcl-2 oncoprotein. Results 16.2% (6/37) hepatocellular carcinoma, 69.2% (9/13) cholangiocarcinoma, 20.0% (1/5) hepatoblastoma, and 22.2% (2 The positive expression rate of bcl-2 oncoprotein in hepatic metastases from /9) was significantly higher than that in paracancerous tissues (P<0.05) and hepatocellular carcinoma (P<0.01). . The positive cells in adjacent tissues were mainly hyperplastic small bile duct epithelial cells, but the positive rate in hepatocellular atypical hyperplasia was rather low (15.4%, 2/13). Conclusion The bcl-2 gene may play an important role in the development of cholangiocarcinoma, but not in the division and proliferation of hepatocytes.