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目的探讨体外受精治疗周期中废弃胚胎的体外发育潜能。方法通过序贯培养法将无原核(0PN)、单个原核(1PN)、多个原核(≥3PN)和卵裂期发育延缓的2原核(2PN)废弃胚胎培养至囊胚期。比较不同受精方式、不同来源胚胎的囊胚形成和d3胚胎的卵裂球数、质量分级等。结果共收集废弃胚胎788个,经序贯培养形成160个囊胚(20.30%),其中38个为优质囊胚(23.75%)。IVF组和ICSI组的囊胚形成率分别为21.17%(141/666)和15.57%(19/122),无统计学差异;1PN胚胎、2PN胚胎、0PN胚胎囊胚形成率较高,但无统计学差异。d3卵裂球数≥6细胞评分Ⅰ-Ⅲ胚胎囊胚形成率较高(P<0.01)。获得的优质囊胚解冻复苏移植4个周期,成功妊娠1例。结论低质量胚胎具有发育至囊胚的潜能,并有成功妊娠的可能,也可成为分离胚胎干细胞的材料来源。
Objective To investigate the in vitro developmental potential of discarded embryos during IVF treatment. Methods Two prokaryotic (2PN) embryos without pro-nuclear (0PN), single pronuclear (1PN), multiple prokaryotic (≥3PN) and cleavage stages were cultured to blastocyst stage by sequential culture. Comparison of different ways of fertilization, blastocyst formation from different embryos and number of blastomeres and quality grading of d3 embryos were compared. Results A total of 788 aborted embryos were collected and 160 blastocysts (20.30%) were obtained by sequential cultivation. Among them, 38 were blastocysts (23.75%). The blastocyst formation rates in IVF group and ICSI group were 21.17% (141/666) and 15.57% (19/122) respectively, with no significant difference. The incidence of blastocyst formation in 1PN embryos, 2PN embryos and 0PN embryos was high, but no Statistical differences. The blastocyst formation rate of Ⅰ-Ⅲ blastocysts with d3 blastomeres≥6were higher (P <0.01). Obtained high-quality embryo thawing and thawing resuscitation 4 cycles, 1 case of successful pregnancy. Conclusion Low quality embryos have the potential to develop into blastocysts and have the potential for successful pregnancy, and may also be the source of materials for isolating embryonic stem cells.