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目的:探讨不同强度的跑步运动对去睾大鼠非负重骨腰椎的骨量和骨显微结构的影响和机制。方法:12周龄SD雄性大鼠40只,随机分为5组,即假手术组(SHAM)、去睾模型组(ORX)、去睾后低强度运动组(ORX+EX1)、去睾后中强度运动组(ORX+EX2)、去睾后高强度运动组(ORX+EX3)。12周后,处死取腰椎骨,做不脱钙骨切片,并测定骨形态计量学各骨量、骨结构、骨代谢参数。各组参数作方差分析,显著性检验。结果:(1)ORX组各参数与SHAM组比较显示:骨量降低(P<0.05),骨显微结构破坏(P<0.05),成骨减弱(P<0.05),破骨活跃(P<0.05)。(2)ORX+EX1和ORX+EX2组各参数与ORX组比较显示:骨量增高(P<0.05),骨显微结构改善(P<0.05),成骨活跃(P<0.05),破骨减弱(P<0.05)。(3)ORX+EX3组各参数与ORX组比较显示:骨量和骨结构均无明显改善(P>0.05),但成骨和破骨均活跃,骨转换加快(P<0.05)。结论:(1)雄性大鼠去睾后能引起骨量丢失及骨结构破坏。(2)低中强度的跑步运动对去睾大鼠不仅能增加骨量还能改善骨显微结构。(3)高强度的跑步运动不能减缓去睾大鼠骨量丢失和骨结构破坏。
OBJECTIVE: To investigate the effects and mechanisms of different intensities of running exercise on the bone mass and bone microstructure of non-weight-bearing bone and lumbar vertebrae of testis in rats. Methods: Forty SD male rats of 12 weeks old were randomly divided into 5 groups: SHAM, ORX, ORX + EX1, Moderate exercise group (ORX + EX2), high strength exercise group (ORX + EX3). Twelve weeks later, lumbar vertebrae were sacrificed and non-decalcified bone slices were obtained. The bone mass, bone structure and bone metabolism parameters of bone morphometry were measured. The parameters of each group were analyzed by variance and significance test. Results: (1) Compared with SHAM group, the parameters of ORX group showed that the bone mass decreased (P <0.05), the bone microstructure was destroyed (P <0.05) 0.05). (2) Compared with ORX group, the parameters of ORX + EX1 and ORX + EX2 group showed that the bone mass increased (P <0.05), the bone microarchitecture improved Weakened (P <0.05). (3) Compared with ORX group, the parameters of ORX + EX3 group showed no significant improvement in bone mass and bone structure (P> 0.05), but the osteogenesis and osteoclast were both active and the bone turnover accelerated (P <0.05). Conclusion: (1) The male rats can cause bone loss and destruction of the bone structure after detoxification. (2) Low-intensity exercise can not only increase bone mass, but also improve bone microstructure in testis rats. (3) High-intensity running exercise can not slow down the bone loss and destruction of bone structure in testis.