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作者曾对生药黄芩(Scutellariae Radix)β-D-葡糖醛酸甙酶(GUS)的纯化等予以报道,此次纯化GUS,在查明其各种性质的同时,探讨了其他生药中是否存在GUS活性。 将中国产干燥生药黄芩(300g)加工成粉末后,用100mM磷酸缓冲液(pH7.0)制成匀浆,分离硫铵(0%～40%),以二乙氨乙基琼脂糖凝胶高流速(DEAE-Sepharose Fast Flow)、PBE~(TM)94聚焦色谱法(Chromatofocusing)以及Sephacry-S-300等提纯出GUS,
The authors reported on the purification of Scutellariae Radix β-D-glucuronidase (GUS). This time, the purified GUS, while ascertaining its various properties, explored the existence of other crude drugs. GUS activity. After drying the Chinese crude drug Astragalus membranaceus (300 g) into a powder, homogenate was made with 100 mM phosphate buffer (pH 7.0) and ammonium sulfate (0% to 40%) was separated into diethylaminoethyl agarose gel. Purification of GUS with high flow rate (DEAE-Sepharose Fast Flow), PBETM94 focused chromatography (Chromatofocusing) and Sephacry-S-300