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从64对EcoRⅠ/MseⅠ引物(其中MseⅠ引物为荧光标记物)中筛选出8对扩增产物多态性高、谱带清晰的引物,对来自美国、荷兰、日本、法国和我国的21个银杏观赏品种的遗传关系进行了研究。结果表明:8对引物共产生1117条谱带,229个特异位点(其中缺失带54条、单态带175条),多态带983条,多态带的比例为88%;每对引物鉴别效率为100%。14个国外银杏观赏品种平均多态带的比例35.86%,7个国内品种平均多态带的比例31.51%。21个观赏品种之间相似系数为0.4899～0.8499。当相似系数为0.7300时,供试观赏品种可分为四类,来源地相同的品种并不单独聚成一类,中国和法国的品种分别属于其中的三类。根据对观赏品种的特异位点、相似系数、聚类结果等进行综合分析表明,‘塔形银杏’、‘垂乳银杏’、‘筒叶银杏’、‘大耳银杏’、‘斑叶银杏’、‘展冠银杏’、‘垂枝银杏’、‘沂源叶籽银杏’这8个品种是银杏观赏品种中的重要特异种质。
Eighty pairs of primers with high polymorphism and clear bands were screened from 64 pairs of EcoRⅠ / MseⅠ primers (MseⅠ primers were fluorescent markers). 21 primers from USA, Netherlands, Japan, France and China The genetic relationship of ornamental species was studied. The results showed that there were 1117 bands and 229 specific sites (of which 54 were absent and 175 were absent) with 8 pairs of primers, and 983 polymorphic bands with 88% of polymorphic bands. Each pair of primers Identification efficiency of 100%. The average polymorphism bands of 14 foreign ginkgo ornamental species were 35.86%, and the average percentage of the seven domestic species was 31.51%. The similarity coefficient of 21 ornamental species was 0.4899 ~ 0.8499. When the similarity coefficient was 0.7300, the ornamental cultivars could be divided into four categories. The cultivars of the same origin were not grouped together, and the varieties of China and France belonged to the three categories. Based on the analysis of the specific loci, similarity coefficient and clustering results of ornamental cultivars, it was found that ’’ gingko ’,’ gingko ’,’ ginkgo ’,’ gingko ’,’ gingko ’ , ’Exhibition crown ginkgo’, ’weeping ginkgo’, ’Yiyuan leaf seed ginkgo’ these eight varieties are ginkgo ornamental species important specific germplasm.