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作者应用两种糖链加工酶抑制剂,即抑制糖蛋白N-糖链合成的地衣霉素及使糖蛋白N-糖链变为高甘露糖型和杂合型的Swainsonine,来研究SMMC-7721人肝癌细胞表面运铁蛋白受体(TR)N-糖链结构的改变对受体再循环过程的影响。实验结果表明,1μg/ml的地衣霉素处理细胞后,细胞生长、蛋白质合成、DNA合成及细胞膜糖蛋白N-糖链的合成均受到不同程度的抑制,但对TR的再循环并无明显影响。2μg/ml的Swainsonine处理细胞后,对细胞生长、蛋白质合成、DNA合成均无明显影响,它使细胞膜糖蛋白N-糖链中甘露糖的掺入增加,并可加快TR再循环过程。
The authors investigated the effects of SMMC-7721, an enzyme inhibitor of two carbohydrate-processing enzymes, clomixamycin, which inhibits the glycolytic N-glycan synthesis, and Swainsonine, which changes the N-glycans of glycoproteins to high mannose and heterozygous types Effect of Alteration of N-Glycan Structure on the Surface of Human Hepatocellular Carcinoma Cells on Re-circulation of Receptors. The results showed that cell growth, protein synthesis, DNA synthesis and cell membrane glycoprotein N-glycan synthesis were inhibited to varying degrees after 1 μg / ml clomomycin treatment, but had no significant effect on the recycle of TR . The treatment with 2μg / ml Swainsonine had no obvious effect on the cell growth, protein synthesis and DNA synthesis. It increased the incorporation of mannose in the N-glycans of cell membrane glycoprotein and accelerated the process of TR recycling.