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目的 :在家兔动脉粥样硬化 (AS)模型上观察肝、主动脉组织及血浆金属硫蛋白 (MT)含量变化 ,认识AS时机体氧化防御系统的改变。方法 :家兔高脂饮食 8周 ,复制高脂血症 -动脉粥样硬化模型 ,分别以[10 9Cd]血红蛋白饱和法和硫代巴比妥酸法测定家兔肝、主动脉组织及血浆MT及丙二醛 (MDA)含量。结果 :AS家兔肝组织和血浆MT水平分别较对照组高 318% (P <0 0 1)和 6 2 % (P <0 0 1) ,而主动脉组织MT含量较对照组低 33 % (P <0 0 1)。肝、主动脉组织及血浆MDA含量分别较对照组高 95 % (P <0 0 1) ,76 % (P <0 0 5 )和 42 % (P <0 0 1) ,肝组织 ,血浆MT变化与MDA变化相关。结论 :AS家兔肝组织和血浆MT产生增多与脂质过氧化损伤有关。
OBJECTIVE: To observe the changes of the content of metallothionein (MT) in liver, aorta and plasma of atherosclerosis (AS) model in rabbits and to understand the change of body’s oxidative defense system in AS. METHODS: Rabbits were given a high-fat diet for 8 weeks to replicate the model of hyperlipidemia-atherosclerosis. [10 9Cd] hemoglobin saturation method and thiobarbituric acid method were used to determine the liver, aortic tissue and plasma MT And malondialdehyde (MDA) content. Results: The levels of MT in liver and plasma in AS rabbits were 318% (P <0.01) and 62% (P <0.01) higher than those in control rabbits, respectively. The MT content in aortic tissues was 33% P <0 0 1). The contents of MDA in liver, aorta and plasma were 95% (P <0.01), 76% (P <0 05) and 42% (P <0.01) And MDA changes related. Conclusion: Increased MT production in liver and plasma of AS rabbits is related to lipid peroxidation injury.